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transcription factor 2 atf2  (Cell Signaling Technology Inc)
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Cell Signaling Technology Inc transcription factor 2 atf2
FIG. 4. Effect of morphine treatment on pneumococcus-induced MyD88-dependent IL-23 production and phosphorylation of IFR3, <t>ATF2,</t> and NF-Bp65. (A) Mouse BMDCs and AMs (1 106) were treated with morphine (1 M) or vehicle for 24 h and then infected with S. pneumoniae for 6 h, and IL-23 concentration was measured in the supernatant by ELISA. **, P 0.01, compared with the vehicle controls. Results are representative of three independent experiments. (B) BMDCs were pretreated with naltrexone (10 M) or vehicle for 1 h before morphine treatment (10 nM to 1 M) and then infected with S. pneumoniae (MOI, 10:1) for 6 h. IL-23 concentration was measured in the supernatant by ELISA. **, P 0.01, compared with the vehicle controls. Results are representative of three independent experiments. (C) BMDCs were pretreated with MyD88 inhibitory or control peptide as described in Materials and Methods and then treated with either morphine (1 M) or vehicle for 24 h and infected with S. pneumoniae for 6 h. The data are presented as mean concentration SEM. **, P 0.05 compared with the vehicle control group; n 6. (D) Levels of p-IRF3 were compared by naïve PAGE and immunoblotting. Phosphorylation of <t>ATF2</t> and NF-Bp65 was compared by Western blot analysis. To verify equality of loading, blots were reprobed with anti--actin or anti-TATA binding protein (TBP). Shown are representative results from one of three independent experiments.
Transcription Factor 2 Atf2, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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enterolert method  (IDEXX)
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IDEXX enterolert method
FIG. 4. Effect of morphine treatment on pneumococcus-induced MyD88-dependent IL-23 production and phosphorylation of IFR3, <t>ATF2,</t> and NF-Bp65. (A) Mouse BMDCs and AMs (1 106) were treated with morphine (1 M) or vehicle for 24 h and then infected with S. pneumoniae for 6 h, and IL-23 concentration was measured in the supernatant by ELISA. **, P 0.01, compared with the vehicle controls. Results are representative of three independent experiments. (B) BMDCs were pretreated with naltrexone (10 M) or vehicle for 1 h before morphine treatment (10 nM to 1 M) and then infected with S. pneumoniae (MOI, 10:1) for 6 h. IL-23 concentration was measured in the supernatant by ELISA. **, P 0.01, compared with the vehicle controls. Results are representative of three independent experiments. (C) BMDCs were pretreated with MyD88 inhibitory or control peptide as described in Materials and Methods and then treated with either morphine (1 M) or vehicle for 24 h and infected with S. pneumoniae for 6 h. The data are presented as mean concentration SEM. **, P 0.05 compared with the vehicle control group; n 6. (D) Levels of p-IRF3 were compared by naïve PAGE and immunoblotting. Phosphorylation of <t>ATF2</t> and NF-Bp65 was compared by Western blot analysis. To verify equality of loading, blots were reprobed with anti--actin or anti-TATA binding protein (TBP). Shown are representative results from one of three independent experiments.
Enterolert Method, supplied by IDEXX, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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FIG. 4. Effect of morphine treatment on pneumococcus-induced MyD88-dependent IL-23 production and phosphorylation of IFR3, ATF2, and NF-Bp65. (A) Mouse BMDCs and AMs (1 106) were treated with morphine (1 M) or vehicle for 24 h and then infected with S. pneumoniae for 6 h, and IL-23 concentration was measured in the supernatant by ELISA. **, P 0.01, compared with the vehicle controls. Results are representative of three independent experiments. (B) BMDCs were pretreated with naltrexone (10 M) or vehicle for 1 h before morphine treatment (10 nM to 1 M) and then infected with S. pneumoniae (MOI, 10:1) for 6 h. IL-23 concentration was measured in the supernatant by ELISA. **, P 0.01, compared with the vehicle controls. Results are representative of three independent experiments. (C) BMDCs were pretreated with MyD88 inhibitory or control peptide as described in Materials and Methods and then treated with either morphine (1 M) or vehicle for 24 h and infected with S. pneumoniae for 6 h. The data are presented as mean concentration SEM. **, P 0.05 compared with the vehicle control group; n 6. (D) Levels of p-IRF3 were compared by naïve PAGE and immunoblotting. Phosphorylation of ATF2 and NF-Bp65 was compared by Western blot analysis. To verify equality of loading, blots were reprobed with anti--actin or anti-TATA binding protein (TBP). Shown are representative results from one of three independent experiments.

Journal: Infection and Immunity

Article Title: Morphine Disrupts Interleukin-23 (IL-23)/IL-17-Mediated Pulmonary Mucosal Host Defense against Streptococcus pneumoniae Infection

doi: 10.1128/iai.00914-09

Figure Lengend Snippet: FIG. 4. Effect of morphine treatment on pneumococcus-induced MyD88-dependent IL-23 production and phosphorylation of IFR3, ATF2, and NF-Bp65. (A) Mouse BMDCs and AMs (1 106) were treated with morphine (1 M) or vehicle for 24 h and then infected with S. pneumoniae for 6 h, and IL-23 concentration was measured in the supernatant by ELISA. **, P 0.01, compared with the vehicle controls. Results are representative of three independent experiments. (B) BMDCs were pretreated with naltrexone (10 M) or vehicle for 1 h before morphine treatment (10 nM to 1 M) and then infected with S. pneumoniae (MOI, 10:1) for 6 h. IL-23 concentration was measured in the supernatant by ELISA. **, P 0.01, compared with the vehicle controls. Results are representative of three independent experiments. (C) BMDCs were pretreated with MyD88 inhibitory or control peptide as described in Materials and Methods and then treated with either morphine (1 M) or vehicle for 24 h and infected with S. pneumoniae for 6 h. The data are presented as mean concentration SEM. **, P 0.05 compared with the vehicle control group; n 6. (D) Levels of p-IRF3 were compared by naïve PAGE and immunoblotting. Phosphorylation of ATF2 and NF-Bp65 was compared by Western blot analysis. To verify equality of loading, blots were reprobed with anti--actin or anti-TATA binding protein (TBP). Shown are representative results from one of three independent experiments.

Article Snippet: Membranes were washed in Tris-buffered saline–Tween 20 (TBST), blocked for 1 h in StartingBlock blocking buffer, and then incubated overnight at 4°C in the primary antibodies, p-NF- Bp65, p-activating transcription factor 2 (ATF2) (Cell Signaling), and interferon response factor 3 (IRF3; Santa Cruz).

Techniques: Phospho-proteomics, Infection, Concentration Assay, Enzyme-linked Immunosorbent Assay, Control, Western Blot, Binding Assay